CLCF1 Protein (AA 28-225)

Catalog No. ABIN7866002

Recombinant CLCF1 protein expressed in Escherichia coli (E. coli).

Quick Overview for CLCF1 Protein (AA 28-225) (ABIN7866002)

Target: CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 28-225

Purpose: Human CLCF1 Protein Recombinant

Sequence: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF

Characteristics:

Protein Size: Recombinant CLCF1 migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human CLCF1 mature chain was expressed in E. Coli. Recombinant Human Cardiotrophin-like cytokine factor 1 encodes amino acid residues 28-225.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: NT-1 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: CLCF1 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))

Alternative Name: CLCF1

Molecular Weight: 22 kDa

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response