CCL5 Protein (AA 25-92)
Quick Overview for CCL5 Protein (AA 25-92) (ABIN7866008)
Target
See all CCL5 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 25-92
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Purpose
- Rat RANTES Protein Recombinant
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Sequence
- SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
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Characteristics
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Protein Size: Recombinant RANTES migrates as an approximately 7 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Rat RANTES mature chain was expressed in E. Coli. Recombinant Rat C-C motif chemokine 5 encodes amino acid residues 25-92.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to chemoattract rat peritoneal macrophages using a concentration range of 50.0-100.0 ng/ml.
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Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) protein (Biotin)
CCL5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) protein (Biotin,His-Avi Tag)CCL5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) protein (His tag,SUMO Tag)CCL5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by Bis-Tris PAGE Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) protein (hFc Tag)CCL5 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) proteinCCL5 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 1-91) protein (GST tag)CCL5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Chemokine (C-C Motif) Ligand 5 (CCL5) protein (His tag)CCL5 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) (Active) protein (His tag)CCL5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) protein (Fc Tag)CCL5 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Chemokine (C-C Motif) Ligand 5 (CCL5) (AA 24-91) protein (Fc Tag)CCL5 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- RANTES was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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Alternative Name
- RANTES
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Molecular Weight
- 7 kDa
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
Target
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