DKK2 Protein (AA 34-259)
Quick Overview for DKK2 Protein (AA 34-259) (ABIN7866015)
Target
See all DKK2 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 34-259
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Purpose
- Human DKK2 Protein Recombinant
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Sequence
- SQIGSSRAKL NSIKSSLGGE TPGQAANRSA GMYQGLAFGG SKKGKNLGQA YPCSSDKECE VGRYCHSPHQ GSSACMVCRR KKKRCHRDGM CCPSTRCNNG ICIPVTESIL TPHIPALDGT RHRDRNHGHY SNHDLGWQNL GRPHTKMSHI KGHEGDPCLR SSDCIEGFCC ARHFWTKICK PVLHQGEVCT KQRKKGSHGL EIFQRCDCAK GLSCKVWKDA TYSSKARLHV CQKI
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Characteristics
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Protein Size: Recombinant DKK2 migrates as an approximately 26 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human DKK2 mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Human Dickkopf-related protein 2 encodes amino acid residues 34-259.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to inhibit IGF-II induced proliferation of MCF-7
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Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (Fc Tag)
DKK2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Dickkopf 2 Homolog (DKK2) (AA 34-100) protein (His-IF2DI Tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (His tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (His-SUMO Tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- DKK2 was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- DKK2 (Dickkopf 2 Homolog (DKK2))
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Alternative Name
- DKK2
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Molecular Weight
- 26 kDa
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Pathways
- WNT Signaling
Target
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