GDF2 Protein (AA 320-429)
Quick Overview for GDF2 Protein (AA 320-429) (ABIN7866027)
Target
See all GDF2 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 320-429
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Purpose
- Human GDF2 Protein Recombinant
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Sequence
- SAGAGSHCQK TSLRVNFEDI GWDSWIIAPK E YEAYECKGGC FFPLADDVTP TK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR
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Characteristics
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Protein Size: Recombinant GDF2 migrates as an approximately 12 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human GDF2 mature chain was expressed in CHO. Recombinant Human Growth/differentiation factor 2 encodes amino acid residues 320-429.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
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Growth Differentiation Factor 2 (GDF2) (AA 1-429) protein (GST tag)
GDF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Growth Differentiation Factor 2 (GDF2) (AA 320-429) protein (His-IF2DI Tag)GDF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Growth Differentiation Factor 2 (GDF2) (AA 320-429) (Active) proteinGDF2 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- GDF2 was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- GDF2 (Growth Differentiation Factor 2 (GDF2))
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Alternative Name
- GDF2
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Molecular Weight
- 12 kDa
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Pathways
- Transition Metal Ion Homeostasis
Target
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