GDF5 Protein (AA 382-501)

Catalog No. ABIN7866029

Recombinant GDF5 protein expressed in Escherichia coli (E. coli).

Quick Overview for GDF5 Protein (AA 382-501) (ABIN7866029)

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 382-501

Purpose: Human GDF5 Protein Recombinant

Sequence: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT

Characteristics:

Protein Size: Recombinant GDF5 migrates as an approximately 21 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human GDF5 mature chain was expressed in E. Coli. Recombinant Human Growth/differentiation factor 5 encodes amino acid residues 382-501.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: GDF5 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Alternative Name: GDF5

Molecular Weight: 21 kDa