Osteopontin Protein (AA 17-314)

Catalog No. ABIN7866058

This Recombinant Osteopontin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Osteopontin Protein (AA 17-314) (ABIN7866058)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 17-314

Purpose: Human SPP1 Protein Recombinant

Sequence: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN

Characteristics:

Protein Size: Recombinant SPP1 migrates as an approximately 33 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human SPP1 mature chain was expressed in E. Coli. Recombinant Human Osteopontin encodes amino acid residues 17-314.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: SPP1 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Molecular Weight: 33 kDa

Pathways: Regulation of Cell Size