TIGAR Protein (AA 1-270)
Quick Overview for TIGAR Protein (AA 1-270) (ABIN7866073)
Target
See all TIGAR ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-270
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Purpose
- Human TIGAR Protein Recombinant
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Sequence
- ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETR
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Characteristics
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Protein Size: Recombinant TIGAR migrates as an approximately 30 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human TIGAR mature chain was expressed in E. Coli. Recombinant Human Fructose-2,6-bisphosphatase TIGAR encodes amino acid residues 1-270.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-269) protein (His tag)
custom-made TIGAR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (His tag)custom-made TIGAR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 1-270) protein (GST tag)TIGAR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
TP53 induced glycolysis regulatory phosphatase (TIGAR) (AA 2-270) protein (His tag)TIGAR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- TIGAR was Lyophilized from PBS, pH 7.2.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Expiry Date
- 24 months
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- TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))
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Alternative Name
- TIGAR
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Molecular Weight
- 30 kDa
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Pathways
- Warburg Effect
Target
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