TWSG1 Protein (AA 2-390)

Catalog No. ABIN7866095

Recombinant TWSG1 protein expressed in Escherichia coli (E. coli).

Quick Overview for TWSG1 Protein (AA 2-390) (ABIN7866095)

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 2-390

Purpose: Human TWSG1 Protein Recombinant

Sequence: CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMNCMF

Characteristics:

Protein Size: Recombinant TWSG1 migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human TWSG1 mature chain was expressed in E. Coli. Recombinant Human Tumor susceptibility gene 101 protein encodes amino acid residues 2-390.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to neutralize BMP-6-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ≤ 2.0 μg/ml of TSG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: TWSG1 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: TWSG1

Molecular Weight: 22 kDa