Osteopontin Protein (AA 17-300)

Catalog No. ABIN7937437

Recombinant Osteopontin protein expressed in NSO Cells.

Quick Overview for Osteopontin Protein (AA 17-300) (ABIN7937437)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: NSO Cells

Purity: > 95 % by SDS-PAGE analyses.

Product Details

Protein Characteristics: AA 17-300

Purpose: Recombinant Human Osteopontin/OPN

Sequence: Ile17-Asn300, with a C-terminal 6-His tag, Accession # NP_000573.1

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x10⁵ cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚,C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.

Application Details

Comment:

Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin & OPN

Molecular Weight: 65 kDa

Pathways: Regulation of Cell Size