FFAR2 Protein (AA 277-330) (His-GST)

Catalog No. ABIN7937643

This Recombinant FFAR2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FFAR2 Protein (AA 277-330) (His-GST) (ABIN7937643)

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: Greater than 85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 277-330

Purification tag / Conjugate: This FFAR2 protein is labelled with His-GST.

Purpose: Recombinant Human FFAR2 protein, partial

Sequence: SSVVRRAFGR GLQVLRNQGS SLLGRRGKDT AEGTNEDRGV GQGEGMPSSD FTTE

Application Details

Comment:

N-terminal 6xHis-GST-tagged

Restrictions: For Research Use only

Handling

Format: Lyophilized or Liquid

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50 %. Customers could use it as reference.

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5 % -50 % glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6 % Trehalose, pH 8.0.

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.

Storage: -20 °C,-80 °C

Storage Comment: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Expiry Date: 6-12 months

Target Details

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Alternative Name: FFAR2

Background:

Relevance: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes. Among SCFAs, the fatty acids containing less than 6 carbons, the most potent activators are probably acetate, propionate and butyrate. Exhibits a SCFA-independent constitutive G protein-coupled receptor activity

Alternative Name(s): G-protein coupled receptor 43

Molecular Weight: 38.1 kDa

UniProt: O15552