YWHAE Protein
Quick Overview for YWHAE Protein (ABIN7938557)
Target
See all YWHAE ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant YWHAE protein
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14-3-3 epsilon (YWHAE) (AA 1-255) protein (GST tag)
YWHAE Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
14-3-3 epsilon (YWHAE) (AA 1-255) proteinYWHAE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
14-3-3 epsilon (YWHAE) protein (GST tag)YWHAE Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by SDS-PAGE
14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)YWHAE Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant YWHAE protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- YWHAE (14-3-3 epsilon (YWHAE))
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Alternative Name
- YWHAE
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Background
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Protein Background: YWHAE (14-3-3 protein epsilon) also known as tyrosine 3-mono-oxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm. Plays a positive role in the antiviral signaling pathway upstream of TBK1 via interaction with RIGI. Mechanistically, directs RIGI redistribution from the cytosol to mitochondrial associated membranes where it mediates MAVS-dependent innate immune signaling during viral infection.
Short Description: Recombinant YWHAE protein that includes full length of human YWHAE protein (accession number NP_006752.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 31.34 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: 14-3-3 protein epsilon, tyrosine 3-mono-oxygenase/tryptophan 5-monooxygenase activation protein, FLJ45465, Epsilon polypeptide
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Pathways
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
Target
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