14-3-3 gamma Protein (YWHAG1)
Quick Overview for 14-3-3 gamma Protein (YWHAG1) (ABIN7938561)
Target
See all 14-3-3 gamma (YWHAG1) ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant YWHAG protein
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14-3-3 gamma (YWHAG1) (AA 1-247) protein (His tag)
custom-made YWHAG1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 gamma (YWHAG1) (AA 1-247) protein (His tag)custom-made YWHAG1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 gamma (YWHAG1) (AA 1-247) protein (GST tag)YWHAG1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
14-3-3 gamma (YWHAG1) (AA 1-247) proteinYWHAG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
14-3-3 gamma (YWHAG1) (AA 2-247) protein (His tag)YWHAG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
14-3-3 gamma (YWHAG1) protein (Myc-DYKDDDDK Tag)YWHAG1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant YWHAG protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- 14-3-3 gamma (YWHAG1)
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Alternative Name
- YWHAG
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Background
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Protein Background: YWHAG (14-3-3 protein gamma) is a adapter protein, Implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24. Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1.
Short Description: Recombinant YWHAG protein that includes full length of human YWHAG protein (accession number NP_036611.2) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 30.47 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: 14-3-3 protein gamma, PPP1R170, Protein kinase C inhibitor protein 1, KCIP-1, EIEE56
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Pathways
- Myometrial Relaxation and Contraction, M Phase
Target
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