14-3-3 zeta Protein (YWHAZ)

Catalog No. ABIN7938564

This Recombinant 14-3-3 zeta protein is produced in Escherichia coli (E. coli).

Quick Overview for 14-3-3 zeta Protein (YWHAZ) (ABIN7938564)

Target: 14-3-3 zeta (YWHAZ)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant YWHAZ protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant YWHAZ protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ

Background:

Protein Background: YWHAZ (14-3-3 protein zeta/delta) is a adapter protein, Implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

Short Description: Recombinant YWHAZ protein that includes full length of human YWHAZ protein (accession number NP_001129171.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 29.91 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: 14-3-3 Protein Zeta/Delta, KCIP, Activation Protein, Delta Polypeptide, YWHAD, HEL4, Protein Kinase C, Inhibitor Protein 1

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location