Arc Protein

Catalog No. ABIN7938568

Recombinant Arc protein expressed in Escherichia coli (E. coli).

Quick Overview for Arc Protein (ABIN7938568)

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant ARC protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ARC protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Alternative Name: ARC

Background:

Protein Background: ARC (Activity-regulated cytoskeleton-associated protein) is a master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1.In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation.

Short Description: Recombinant ARC protein that includes full length of the human ARC protein (accession number NP_056008.1) was expressed in E. coli cells with an N-terminal His tag with an observed molecular weight of 47.48 kDa.

Aliases: Activity-regulated cytoskeleton-Associated protein, KIAA0278, ARC, ARG3.1, HArc, ARG3.1