ADAR Protein

Catalog No. ABIN7938571

This Recombinant ADAR protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for ADAR Protein (ABIN7938571)

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant ADAR1 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ADAR1 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: ADAR1

Background:

Protein Background: ADAR1 (Double-stranded RNA-specific adenosine deaminase), Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine, pre-mRNA splicing by altering splice site recognition sequences, RNA stability by changing sequences involved in nuclease recognition, genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication, and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing).

Short Description: Recombinant ADAR1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001102.3) with an N-terminal FLAG tag. The molecular weight of the protein is 137.34 kDa.

Aliases: DRADA, Double Stranded RNA -Specific Adenosine Deaminase, G1P1, IFI4, Interferon Inducible Protein 4, DSRAD, IFI-4, P136, DSH

Pathways: Protein targeting to Nucleus