ADAR Protein (AA 1-295)

Catalog No. ABIN7938572

This Recombinant ADAR protein is produced in Escherichia coli (E. coli).

Quick Overview for ADAR Protein (AA 1-295) (ABIN7938572)

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1-295

Purpose: Recombinant ADAR1 (1-295) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ADAR1 (1-295) protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: ADAR1

Background:

Protein Background: ADAR1 (Double-stranded RNA-specific adenosine deaminase), Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine, pre-mRNA splicing by altering splice site recognition sequences, RNA stability by changing sequences involved in nuclease recognition, genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication, and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing).

Short Description: Recombinant ADAR1 (1-295) protein that includes amino acids 1-295 of human ADAR1 protein (accession number NP_001102.3) was expressed in E. coli cells with an N-terminal 6xHis and Flag tag. The molecular weight of the protein is 35.88 kDa.

Aliases: DRADA, Double Stranded RNA -Specific Adenosine Deaminase, G1P1, IFI4, Interferon Inducible Protein 4, DSRAD, IFI-4, P136, DSH

Pathways: Protein targeting to Nucleus