AS3MT Protein
Quick Overview for AS3MT Protein (ABIN7938607)
Target
See all AS3MT ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant AS3MT protein
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Arsenic (+3 Oxidation State) Methyltransferase (AS3MT) (AA 1-375) protein (His tag)
custom-made AS3MT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Arsenic (+3 Oxidation State) Methyltransferase (AS3MT) (AA 1-375) protein (His-IF2DI Tag)AS3MT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Arsenic (+3 Oxidation State) Methyltransferase (AS3MT) (AA 1-375) protein (His tag)AS3MT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Arsenic (+3 Oxidation State) Methyltransferase (AS3MT) protein (Myc-DYKDDDDK Tag)AS3MT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant AS3MT protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- AS3MT (Arsenic (+3 Oxidation State) Methyltransferase (AS3MT))
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Alternative Name
- AS3MT
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Background
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Protein Background: Methylarsonite methyltransferase is also known as AS3MT,and the main function of AS3MT is catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals .It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2 .Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH.
Short Description: Recombinant AS3MT protein was expressed in E.coli as the full length protein (accession number NP_065733.2) with an N-terminal 6xHis tag. The molecular weight of the protein is 43.68 kDa.
Aliases: Arsenite Methyltransferase, Methylarsonite Methyltransferase
Target
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