CDC42BPA Protein (AA 1-473)

Catalog No. ABIN7938683

This Recombinant CDC42BPA protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CDC42BPA Protein (AA 1-473) (ABIN7938683)

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1-473

Purpose: Recombinant MRCKa / CDC42BPA (1-473) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MRCKa / CDC42BPA (1-473) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Alternative Name: MRCKa & CDC42BPA

Background:

Protein Background: CDC42BPA (CDC42 Binding Protein Kinase Alpha) is a serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. This protein regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, CDC42BPA is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. In concert with FAM89B/LRAP25 CDC42BPA mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation.

Short Description: Recombinant MRCKa / CDC42BPA (1-473) protein that includes amino acids 1-473 of human MRCKa protein (accession number NP_001352948.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 55.7 kDa.

Aliases: CDC42 Binding Protein Kinase Alpha