CDK1/CyclinB1 (Complex) Protein

Catalog No. ABIN7938686

This Recombinant CDK1/CyclinB1 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for CDK1/CyclinB1 (Complex) Protein (ABIN7938686)

Target: CDK1/CyclinB1

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant CDK1 / Cyclin B1 Complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant CDK1 / Cyclin B1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDK1/CyclinB1

Alternative Name: CDK1 & Cyclin B1

Background:

Protein Background: The CDK1 / Cyclin B1 complex is a master regulator of the G2/M transition and progression through mitosis in eukaryotic cells. As a cyclin-dependent kinase complex, it phosphorylates numerous substrates to drive critical mitotic events, including nuclear envelope breakdown, chromosome condensation, spindle assembly, and activation of the anaphase-promoting complex/cyclosome (APC/C) for sister chromatid separation. Beyond its canonical cell cycle role, CDK1 / Cyclin B1 localizes to mitochondria and phosphorylates complex I proteins to enhance ATP production, thereby coupling energy generation with division demands. It also maintains chromosomal stability by regulating kinetochore microtubule attachments and spindle checkpoint signaling through phosphorylation of Mps1 and Aurora B kinases. In oocyte meiosis, the complex is essential for the metaphase I-to-anaphase I transition by promoting APC/C activation and homologous chromosome segregation. Dysregulation of CDK1 / Cyclin B1 contributes to cancer pathogenesis, as its overexpression or defective function (e.g., impaired Cyclin B1 induction) is linked to tumor proliferation, chemoresistance (e.g., T-DM1 in breast cancer), and genomic instability, making it a significant therapeutic target.

Short Description: Recombinant CDK1 / Cyclin B1 Complex that includes full length human CDK1 protein (accession number NP_001777.1) with an C-terminal His tag and full length human Cyclin B1 protein (accession number NP_114172.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK1 and Cyclin B1 are 35.73 kDa and 50.03 kDa, respectively.