CDK2/CyclinA2 (Complex) Protein

Catalog No. ABIN7938690

This Recombinant CDK2/CyclinA2 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for CDK2/CyclinA2 (Complex) Protein (ABIN7938690)

Target: CDK2/CyclinA2

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant CDK2 / Cyclin A2 Complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant CDK2 / Cyclin A2 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDK2/CyclinA2

Alternative Name: CDK2 & Cyclin A2

Background:

Protein Background: CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. CDK2 / Cyclin A2 complex phosphorylate proteins like pocket proteins (Rb, p107, p130) and proteins involved in DNA synthesis, thereby driving S-phase progression. In line with its role in regulating S-phase and as a typical E2F target gene, cyclin A2 expression is induced upon entry into S-phase, persists through the S- and G2 phases, and is degraded upon entry into mitosis.

Short Description: Recombinant CDK2 / Cyclin A2 Complex that includes full length human CDK2 protein (accession number NP_001789.2) with an C-terminal His tag and full length human Cyclin A2 protein (accession number NP_001228.2) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK2 and Cyclin A2 are 35.57 kDa and 50.24 kDa, respectively.