CDK2/CyclinE1 (Complex) Protein

Catalog No. ABIN7938691

Recombinant CDK2/CyclinE1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CDK2/CyclinE1 (Complex) Protein (ABIN7938691)

Target: CDK2/CyclinE1

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant CDK2 / Cyclin E1 Complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant CDK2 / Cyclin E1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDK2/CyclinE1

Alternative Name: CDK2 & Cyclin E1

Background:

Protein Background: CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. Cyclin E/ CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase.

Short Description: Recombinant CDK2 / Cyclin E1 Complex that includes full length human CDK2 protein (accession number NP_001789.2) with a C-terminal His tag and full-length human Cyclin E1 protein (accession number NP_001229.1) with a C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK2 and Cyclin E1 are 35.57 kDa and 48.77 kDa, respectively.