CDK4/CyclinD1 (Complex) Protein

Catalog No. ABIN7938694

Recombinant CDK4/CyclinD1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CDK4/CyclinD1 (Complex) Protein (ABIN7938694)

Target: CDK4/CyclinD1

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant CDK4 / Cyclin D1 Complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant CDK4 / Cyclin D1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CDK4/CyclinD1

Alternative Name: CDK4 & Cyclin D1

Background:

Protein Background: Cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Short Description: Recombinant CDK4 / Cyclin D1 Complex that includes full-length human CDK4 protein (accession number NP_000066.1) with an N-terminal His tag and full-length human Cyclin D1 protein (accession number NP_444284.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK4 and Cyclin D1 are 36.83 kDa and 35.42 kDa, respectively.