CARM1 Protein (full length)

Catalog No. ABIN7938720

This Recombinant CARM1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for CARM1 Protein (full length) (ABIN7938720)

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: full length

Purpose: Recombinant PRMT4 (CARM1) protein

Characteristics: Recombinant human PRMT4 (CARM1) was expressed in a baculovirus expression system as the full-length protein (accession number NP_954592.1) with an N-terminal Flag tag. The molecular weight of the protein is 66.8 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Full length recombinant PRMT4 (CARM1) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100 and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Alternative Name: PRMT4

Background:

Protein Background: PRMT4 (CARM1) (Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. PRMT4 (CARM1) methylates histone H3 at Arg2, Arg17 and Arg26, it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

Short Description: Recombinant human PRMT4 (CARM1) was expressed in a baculovirus expression system as the full length protein (accession number NP_954592.1) with an N-terminal Flag tag. The molecular weight of the protein is 66.8 kDa.

Aliases: Coactivator Associated Arginine Methyltransferase 1, Protein Arginine N-Methyltransferase 4

Molecular Weight: 66.8 kDa

NCBI Accession: NP_954592

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation