CRISPR-Cas9 (full length) protein (His tag)

Catalog No. ABIN7938731

This Recombinant CRISPR-Cas9 protein is produced in Escherichia coli (E. coli).

Quick Overview for CRISPR-Cas9 (full length) protein (His tag) (ABIN7938731)

Target: CRISPR-Cas9

Protein Type: Recombinant

Origin: Streptococcus pyogenes

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: full length

Purification tag / Conjugate: His tag

Purpose: Recombinant Cas9 protein

Characteristics: Recombinant Cas9 protein was expressed in E. coli as the full-length protein (accession number WP_010922251.1) with an N-terminal 6xHis Tag and a C-terminal nuclear localization sequence. The molecular weight of the protein is 161.2 kDa. This protein is useful for the study of in vitro and in vivo DNA endonuclease reactions as well as other genome engineering.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Cas9 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: CRISPR-Cas9

Alternative Name: Cas9

Background:

Protein Background: Type II CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) RNA-guided endonuclease Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease associated with adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking for sites complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. These breaks can lead to gene inactivation or the introduction of heterologous genes through non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Short Description: Recombinant Cas9 protein was expressed in E.coli as the full length protein (accession number WP_010922251.1) with an N-terminal 6x His tag and a C-terminal nuclear localization sequence. The molecular weight of the protein is 161.2 kDa.

Aliases: Clustered Regularly Interspersed Short Palindromic Repeasts, CRISPR associated protein 9

Molecular Weight: 161.2 kDa

NCBI Accession: WP_010922251