DDX54 Protein

Catalog No. ABIN7938756

This Recombinant DDX54 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for DDX54 Protein (ABIN7938756)

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant DDX54 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant DDX54 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Alternative Name: DDX54

Background:

Protein Background: DDX54 (ATP-dependent RNA helicase DDX54) is a nucleolar DEAD-box RNA helicase that functions as a key regulator of transcription and ribosome biogenesis. It is recruited to gene promoters, including those of nuclear receptors and stress-responsive genes, where it acts as a transcriptional co-regulator to modulate their expression. Furthermore, DDX54 plays a direct role in the nucleolar stages of ribosomal RNA (rRNA) processing and maturation, which is essential for the proper assembly of ribosomes. Its activity is also implicated in the cellular stress response, where it contributes to the reorganization of nucleolar architecture and halts rRNA synthesis to conserve energy. Dysregulation of DDX54 has been associated with cancers, underscoring its importance in fundamental cell growth and proliferation pathways.

Short Description: Recombinant DDX54 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001104792.1) with an N-terminal FLAG tag. The molecular weight of DDX54 is 99.94 kDa.

Aliases: ATP-dependent RNA helicase DDX54, DP97, DEAD-Box Helicase 54

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway