EMG1 Protein

Catalog No. ABIN7938792

This Recombinant EMG1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for EMG1 Protein (ABIN7938792)

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EMG1 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EMG1 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 10 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EMG1 (EMG1 Nucleolar Protein Homolog (EMG1))

Alternative Name: EMG1

Background:

Protein Background: Ribosomal RNA small subunit methyltransferase NEP1 (EMG1) is a highly conserved nucleolar protein with an essential function in ribosome biogenesis. A mutation in the human Nep1 homolog causes Bowen-Conradi syndrome-a severe developmental disorder. Structures of Nep1 revealed a dimer with a fold similar to the SPOUT-class of RNA-methyltransferases suggesting that Nep1 acts as a methyltransferase in ribosome biogenesis. The target for this putative methyltransferase activity has not been identified yet.

Short Description: Recombinant EMG1 protein was expressed in E.coli as the full length protein (accession number NP_006322.4) with an N-terminal 6xHis tag. The molecular weight of the protein is 28.52 kDa.

Aliases: Ribosomal RNA small subunit methyltransferase NEP1