EEF1A2 Protein

Catalog No. ABIN7938818

Recombinant EEF1A2 protein expressed in Escherichia coli (E. coli).

Quick Overview for EEF1A2 Protein (ABIN7938818)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EEF1A2 protein

Characteristics: EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2), also called as EF1A or Statin, is an isoform of the alpha subunit of the elongation factor-1 complex. This complex is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. There are two isoforms for subunit alpha, EEF1A1 and EEF1A2. EEF1A2 promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EEF1A2 protein is supplied in 25 mM Tris-HCl pH 7.4, 500 mM NaCl, 10 % glycerol, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Alternative Name: EEF1A2

Background:

Protein Background: EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2), also called as EF1A or Statin, is an isoform of the alpha subunit of the elongation factor-1 complex. This complex is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. There are two isoforms for subunit alpha, EEF1A1 and EEF1A2. EEF1A2 promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.

Short Description: Recombinant EEF1A2 protein was expressed in E.coli as the full length protein (accession number NP_001949.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 54 kDa.

Aliases: Eukaryotic Translation Elongation Factor 1 Alpha 2