EEF2 Protein

Catalog No. ABIN7938824

Recombinant EEF2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for EEF2 Protein (ABIN7938824)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EEF2 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Recombinant EEF2 protein is in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2

Background:

Protein Background: EEF2 (Elongation factor 2) promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Mostly eEF2 is regulated by phosphorylation and dephosphorylation by a specific kinase known as eEF2 kinase, which itself is up regulated by various mechanisms in the eukaryotic cell. The activity of this kinase is dependent on calcium ions and calmodulin. Recently it has been shown that the activity of eEF2 kinase is regulated by MAP kinase signalling and mTOR signalling pathway.

Short Description: Recombinant EEF2 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001952.1) with an N-terminal FLAG tag. The molecular weight of EEF2 is 96.91 kDa.

Aliases: EF2, Elongation Factor 2, EF-2, Epididymis Secretory Binding Protein

Pathways: AMPK Signaling