EIF4A1 Protein

Catalog No. ABIN7938829

Recombinant EIF4A1 protein expressed in Escherichia coli (E. coli).

Quick Overview for EIF4A1 Protein (ABIN7938829)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EIF4A1 protein

Characteristics: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EIF4A1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Alternative Name: EIF4A1

Background:

Protein Background: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is a ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.

Short Description: Recombinant EIF4A1 protein was expressed in E.coli as the full length protein (accession number NP_001407.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.7 kDa.

Aliases: Eukaryotic Translation Initiation Factor 4A1, DDX2A