EIF4A2 Protein

Catalog No. ABIN7938832

This Recombinant EIF4A2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for EIF4A2 Protein (ABIN7938832)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EIF4A2 protein

Characteristics: EIF4A2 (Eukaryotic Translation Initiation Factor 4A, Isoform 2), also called EIF4F or DDX2B, is an ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to the ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. Diseases associated with EIF4A2 include Retinitis Pigmentosa 29 and X-Linked Hereditary Ataxia. Among its related pathways are the TGFb pathway and Apoptotic pathways in Synovial Fibroblasts.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EIF4A2 protein is supplied in 25 mM Tris-HCl pH 8.0, 500 mM NaCl, 10 % glycerol, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background:

Protein Background: EIF4A2 (Eukaryotic Translation Initiation Factor 4A, Isoform 2), also called EIF4F or DDX2B, is an ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to the ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. Diseases associated with EIF4A2 include Retinitis Pigmentosa 29 and X-Linked Hereditary Ataxia. Among its related pathways are the TGFb pathway and Apoptotic pathways in Synovial Fibroblasts.

Short Description: Recombinant EIF4A2 protein was expressed in E.coli as the full length protein (accession number NP_001958.2) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.9 kDa.

Aliases: Eukaryotic Translation Initiation Factor 4A2, DDX2B