EIF4A3 Protein

Catalog No. ABIN7938833

This Recombinant EIF4A3 protein is produced in Escherichia coli (E. coli).

Quick Overview for EIF4A3 Protein (ABIN7938833)

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant EIF4A3 protein

Characteristics: EIF4A3 (Eukaryotic Translation Initiation Factor 4A, Isoform 3), also called as DDX48, NUK34 or RCPS, is a ATP-dependent RNA helicase, which is involved in pre-mRNA splicing as component of the spliceosome. It is a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The RNA-dependent ATPase and RNA-helicase activities of EIF4A3 are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. This protein is involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. EIF4A3 binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. And it shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant EIF4A3 protein is supplied in 25 mM Tris-HCl pH 7.4, 300 mM NaCl,5 % glycerol, 0.1 % Triton X-100.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: EIF4A3

Background:

Protein Background: EIF4A3 (Eukaryotic Translation Initiation Factor 4A, Isoform 3), also called as DDX48, NUK34 or RCPS, is a ATP-dependent RNA helicase, which is involved in pre-mRNA splicing as component of the spliceosome. It's a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The RNA-dependent ATPase and RNA-helicase activities of EIF4A3 are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. This protein is involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. EIF4A3 binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. And it shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.

Short Description: Recombinant EIF4A3 protein was expressed in E.coli as the full length protein (accession number NP_055555.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 50.4 kDa.

Aliases: Eukaryotic Translation Initiation Factor 4A3, DDX48