Fibrillarin Protein (FBL)
Quick Overview for Fibrillarin Protein (FBL) (ABIN7938842)
Target
See all Fibrillarin (FBL) ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant FBL protein
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Fibrillarin (FBL) (AA 1-321) protein (GST tag)
FBL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibrillarin (FBL) (AA 83-321) protein (His tag)FBL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibrillarin (FBL) protein (Myc-DYKDDDDK Tag)FBL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant FBL protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Fibrillarin (FBL)
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Alternative Name
- FBL
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Background
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Protein Background: FBL(rRNA 2'-O-methyltransferase fibrillarin) is a S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins.Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA.Site specificity is provided by a guide RNA that base pairs with the substrate.Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA .Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes.U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity .Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.
Short Description: Recombinant FBL protein that includes full length of human FBL protein (accession number NP_001427.2) was expressed was expressed in E. coli cells with an N-terminal GST tag. The molecular weight of the protein is 60.62 kDa.
Aliases: rRNA 2'-O-methyltransferase fibrillarin, FLRN, RNU3IP1, Nop1, FIB, EC 2.1.1.37
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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