K-RAS Protein (AA 1-186, G12V)
Quick Overview for K-RAS Protein (AA 1-186, G12V) (ABIN7938882)
Target
See all K-RAS (KRAS) ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-186, G12V
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Purpose
- Recombinant KRAS (1-186, G12V) protein
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GTPase Kras (KRAS) (AA 16-125) protein (GST tag)
KRAS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
GTPase Kras (KRAS) (AA 1-188) protein (GST tag)KRAS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
GTPase Kras (KRAS) protein (His tag)KRAS Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
GTPase Kras (KRAS) (AA 2-185) protein (Fc Tag)KRAS Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
GTPase Kras (KRAS) (AA 2-185) protein (His tag)KRAS Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
GTPase Kras (KRAS) (AA 2-185) protein (mFc Tag)KRAS Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
GTPase Kras (KRAS) (AA 1-189) protein (GST tag)KRAS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant KRAS (1-186, G12V) protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- K-RAS (KRAS) (GTPase Kras (KRAS))
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Alternative Name
- KRAS
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Background
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Protein Background: KRAS (GTPase KRas) protein is a GDP/GTP binding protein, and the more important genes of the same family include HRAS and NRAS. KRAS is activated when combined with GTP and closed when combined with GDP. KRAS can be transiently activated by growth factors or tyrosine kinases (such as EGFR). The activated KRAS can activate downstream signals such as PI3K-AKT-mTOR that control cell production pathway, and the RAS-RAF-MEK-ERK signaling pathway that controls cell proliferation. Mutant KRAS will continue to be activated even without activation of kinases such as EGFR, resulting in continued cell proliferation and eventually canceration. KRAS mutations are found in a variety of tumors, the most common ones include lung cancer and pancreatic cancer.
Short Description: Recombinant KRAS (1-186, G12V) protein that includes amino acids 1-186 of human KRAS protein (accession number NP_203524.1) was expressed in E. coli cells with a G12V mutation and a C-terminal 6xHis tag. The molecular weight of the protein is 22.36 kDa.
Aliases: GTPase KRas, KRAS1, KRAS2, Kirsten Rat Sarsoma Viral Oncogene Homolog, C-Ki-Ras, KRAS3B, K-Ras P21 Protein
Target
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