Histone H2A Protein (His tag)

Catalog No. ABIN7939015

Recombinant Histone H2A protein expressed in Escherichia coli (E. coli).

Quick Overview for Histone H2A Protein (His tag) (ABIN7939015)

Target: Histone H2A

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This Histone H2A protein is labelled with His tag.

Purpose: Recombinant Histone H2A (Human)

Characteristics: Recombinant Histone H2A (Human) (accession number NP_003503.1) was expressed in E. coli. This protein does not contain any tag, it has an observed molecular weight of 14.1 kDa. The recombinant histone is >90 % pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Histone H2A (Human) is supplied in 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 5 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone H2A

Background:

Protein Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Short Description: Recombinant Histone H2A (Human) (accession number NP_003503.1) was expressed in E. coli cells. This protein does not contain any tag and has a molecular weight of 14.1 kDa.

Molecular Weight: 14.1 kDa

NCBI Accession: NP_003503