Histone H2B Protein (His tag)

Catalog No. ABIN7939017

This Recombinant Histone H2B protein is expressed in Escherichia coli (E. coli).

Quick Overview for Histone H2B Protein (His tag) (ABIN7939017)

Target: Histone H2B

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Enzyme Kinetics (EK), Screening Assay (ScA)

Product Details

Purification tag / Conjugate: This Histone H2B protein is labelled with His tag.

Purpose: Recombinant Histone H2B (Human)

Characteristics: Recombinant Histone H2B (Human) (accession number NP_003509.1) was expressed in E. coli. This protein does not contain any tag, it has an observed molecular weight of 13.9 kDa. The recombinant protein is >85 % pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Recombinant Histone H2B (Human) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Histone H2B (Human) was expressed in E.coli and is supplied in 25 mM Tris pH 7.40, 150 mM NaCl, and 5 % Glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone H2B

Background:

Protein Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Short Description: Recombinant Histone H2B (Human) (accession number NP_003509.1) was expressed in E. coli cells. This protein does not contain any tag with a molecular weight of 13.9 kDa.

Molecular Weight: 13.9 kDa

NCBI Accession: NP_003509