Histone 3 Protein (H3K4me3, Nucleosome)

Catalog No. ABIN7939073

This Recombinant Histone 3 protein is produced in Escherichia coli (E. coli).

Quick Overview for Histone 3 Protein (H3K4me3, Nucleosome) (ABIN7939073)

Target: Histone 3 (H3) (Histone H3 (H3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Screening Assay (ScA), Enzyme Kinetics (EK)

Product Details

Protein Characteristics: H3K4me3, Nucleosome

Purpose: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated

Characteristics: Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Purification: purified using FPLC.

Application Details

Application Notes: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Mononucleosomes H3K4me3 - biotinylated (20 μg protein + 20 μg DNA) are supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT and 20 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background:

Protein Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.

Short Description: Recombinant Mononucleosomes H3K4me3 (EPL) - biotinylated, consist of 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end) (accession number NP_066403.2) with trimethylation at lysine 4, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is ~108 kDa. H3K4me3 (Histone H3 trimethyl Lys4) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing trimethyl lysine 4 via a native peptide bond.

Aliases: methylated Histone, H3K4 tri-methylated, EPL Histone