Histone 3 Protein (H3K36me3, Nucleosome, pseudo-methylated)

Catalog No. ABIN7939087

This Recombinant Histone 3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Histone 3 Protein (H3K36me3, Nucleosome, pseudo-methylated) (ABIN7939087)

Target: Histone 3 (H3) (Histone H3 (H3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Product Details

Protein Characteristics: H3K36me3, Nucleosome, pseudo-methylated

Purpose: Recombinant Mononucleosomes H3K36me3 (MLA)

Characteristics: Mononucleosomes H3K36me3 (MLA) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 trimethyl Lys36 (H3K36me3) (MLA) that includes amino acids 1-136 (end) (accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli. The molecular weight of histone octamer is 108 kDa.

Application Details

Application Notes: Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatinin vitro.

Comment:

Pseudomethylated Lysines have been generated using the partented Methylated Lysin Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine gamma-methylene. The MLA technique provides precise control over the site and degree of methylation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Mononucleosomes H3K36me3 (MLA) (20 μg protein + 20 μg DNA) are supplied in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT, 20 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background:

Protein Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine Upsilon-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

Short Description: Mononucleosomes H3K36me3 (MLA) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 trimethyl Lys36 (H3K36me3) (MLA) that includes amino acids 1-136 (end) (accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa.

Molecular Weight: 108 kDa

NCBI Accession: NP_003520