Histone H3.1 Protein (HIST1H3B) (Nucleosome, R26P) (Biotin)

Catalog No. ABIN7939144

This Recombinant Histone H3.1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Histone H3.1 Protein (HIST1H3B) (Nucleosome, R26P) (Biotin) (ABIN7939144)

Target: Histone H3.1 (HIST1H3B)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Product Details

Protein Characteristics: Nucleosome, R26P

Purification tag / Conjugate: This Histone H3.1 protein is labelled with Biotin.

Purpose: Recombinant Mononucleosomes H3.1 (R26P) - biotin

Characteristics: Recombinant Mononucleosomes H3.1 (R26C) - biotin consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.1 that includes amino acids 1-136 (end) (accession number NM_003529) with a point mutation Arg26Pro, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of the histone octamer is 108 kDa. Recombinant Mononucleosomes H3.1 (R26P) - biotin are suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

Application Details

Application Notes: Recombinant Mononucleosomes H3.1 (R26P) - biotin are suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Mononucleosomes H3.1 (R26P) - biotin (20 μg protein + 20 μg DNA) is supplied in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT, and 20 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1

Background:

Protein Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are signifcantly more active, as well as specifc, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. Histones are linked to tumorigenesis primarily through alterations in their PTMs and the enzymes regulating these modifications, suggesting that they might disrupt the reading, writing, and/or erasing of these marks. Mutations in histone H3 occur with high genetic penetrance within rare paediatric gliomas and sarcomas. In H3 variants, the mutation is most often a lysine-to-methionine (K-M) mutation, occasionally glycine mutations (G34R/V/W/L) occur too. More K-to-M/I mutations were observed, raising the possibility that the functional effects associated with known K-to-M/I changes (that is, function in a dominant fashion to block the methylation of corresponding lysines on wild type histones) may extend to additional contexts. Researchers found that mutations in the subset with a TMB ≤ 2 mutations per Mb included H3 (K27M) and H3 (G34W), and other mutations like H3 (E105K/Q), mutations at H3 N-terminal residues at or near PTM sites including R2, R8, K18 and R26, as well as residues in the acidic patch such as H2A residues E56, E64, E9, and E92 and H2B residues E105 and E133, which might act as oncohistones.

Short Description: Recombinant Mononucleosomes H3.1 (R26C) - biotin consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.1 that includes amino acids 1-136 (end) (accession number NM_003529) with a point mutation Arg26Pro, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of the histone octamer is 108 kDa.

Molecular Weight: 108 kDa