Histone H4 Protein (HISTH4) (His tag)
Quick Overview for Histone H4 Protein (HISTH4) (His tag) (ABIN7939153)
Target
See all Histone H4 (HISTH4) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Histone H4 protein is labelled with His tag.
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Purpose
- Recombinant Histone H4, His-Tag (Human)
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Characteristics
- Recombinant Histone H4, His-Tag (Human) (accession number NP_003539.1) was expressed in E. coli and contains a C-terminal 6xHis Tag with a molecular weight of 12.19 kDa. The recombinant histone H4 is >85 % pure by SDS-PAGE. It is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.
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Histone H4 (HISTH4) (H4K5me3), (pseudo-methylated) protein (His tag)
HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
Histone H4 (HISTH4) (H4K16me1), (pseudo-methylated) protein (His tag)HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
Histone H4 (HISTH4) (H4K16me2), (pseudo-methylated) protein (His tag)HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
Histone H4 (HISTH4) (H4K5me1), (pseudo-methylated) protein (His tag)HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
Histone H4 (HISTH4) (H4K5me2), (pseudo-methylated) protein (His tag)HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
Histone H4 (HISTH4) protein (His tag)HISTH4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Histone H4 (HISTH4) protein (Myc-DYKDDDDK Tag)HISTH4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Histone H4 (HISTH4) (H4K20me3), (pseudo-methylated) protein (His tag)HISTH4 Origin: Xenopus laevis Host: Escherichia coli (E. coli) Recombinant >98 % pure by SDS-PAGE PC, S
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Full length recombinant Histone H4, His-Tag (Human) is supplied in 25 mM Tris pH 7.5, 150 mM NaCl, and 20 % Glycerol.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Histone H4 (HISTH4)
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Alternative Name
- Histone H4
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Background
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Protein Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
Short Description: Recombinant Histone H4, His-Tag (Human) (accession number NP_003539.1) was expressed in E. col cells and contains a C-terminal 6xHis Tag with a molecular weight of 12.19 kDa. The recombinant histone H4 is >95 % pure by SDS-PAGE.
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Molecular Weight
- 12.19 kDa
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NCBI Accession
- NP_003539
Target
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