HIPK3 Protein (AA 163-562)

Catalog No. ABIN7939163

This Recombinant HIPK3 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for HIPK3 Protein (AA 163-562) (ABIN7939163)

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 163-562

Purpose: Recombinant HIPK3 (163-562) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant HIPK3 (163-562) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Alternative Name: HIPK3

Background:

Protein Background: HIPK3 (Homeodomain Interacting Protein Kinase 3), also known as FIST. It is a serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression, HIPK3 phosphorylates JUN and RUNX2. It seems to negatively regulate apoptosis by promoting FADD phosphorylation and enhances androgen receptormediated transcription. It may also act as a transcriptional co-repressor for NK homeodomain transcription factors. In osteoblasts, HIPK3 supports transcription activation and phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2 mediated activation of the osteocalcin FGF-responsive element (OCFRE).

Short Description: Recombinant HIPK3 (163-562) protein that includes amino acids 163-562 of human HIPK3 protein (accession number NP_005725.3) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 46.6 kDa.

Aliases: Homeodomain Interacting Protein Kinase 3, DYRK6, FIST3, YAK1