ITK Protein (AA 352-620)

Catalog No. ABIN7939168

This Recombinant ITK protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for ITK Protein (AA 352-620) (ABIN7939168)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 352-620

Purpose: Recombinant ITK (352-620) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ITK (352-620) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK

Background:

Protein Background: ITK (IL2 Inducible T Cell Kinase) is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response and regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. The phosphorylation leads to ITK autophosphorylation and full activation. Once activated, it phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. It also phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Diseases associated with ITK include Lymphoproliferative Syndrome 1 and Agammaglobulinemia, X-Linked.

Short Description: Recombinant ITK (352-620) protein that includes amino acids 352-620 of human ITK protein (accession number NP_005537.3) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 31.8 kDa.

Aliases: IL-2 Inducible T-Cell Kinase, Tyrosine-Protein Kinase LYK, EMT, LYK, LPFS1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway