Kdm6b Protein (AA 1043-1682)

Catalog No. ABIN7939251

This Recombinant Kdm6b protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Kdm6b Protein (AA 1043-1682) (ABIN7939251)

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Enzyme Kinetics (EK), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1043-1682

Purpose: Recombinant JMJD3 / KDM6B (1043-1682) protein

Characteristics: Recombinant JMJD3 / KDM6B (1043-1682) includes amino acids 1043-1682 of human JMJD3 / KMD6B protein (accession number NP_001073893.1), it was expressed in a baculovirus expression system and contains a C-terminal Flag-Tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90 % pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Recombinant JMJD3 / KDM6B (1043-1682) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HDM Activity Assay Conditions: 1 uM H3K27me3 peptide was incubated with different concentrations of JMJD3 (1043- 1682) protein in 10 µL reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.02 % Triton X-100, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2•6H2O for 2 hours, then 10 micro,l H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Restrictions: For Research Use only

Handling

Buffer: Recombinant JMJD3 / KDM6B (1043-1682) protein is supplied at a concentration of 0.4 μg/ul in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5 % glycerol, 0.04 % Triton X-100, 0.2 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Alternative Name: JMJD3 & KDM6B

Background:

Protein Background: JMJD3 / KDM6B (Jumonji domain-containing protein 3 lysine (K)-specific demethylase 6B) is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby, playing a central role in histone code. JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27'. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 / KDM6B is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

Short Description: Recombinant JMJD3 / KDM6B (1043-1682) that includes amino acids 1043 - 1682 of human JMJD3 / KDM6B protein (accession number NP_001073893.1) was expressed in Baculovirus expression system and contains a C-terminal Flag tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90 % pure by SDS-PAGE.

Aliases: Jumonji Domain Containing 3 Histone Lysine Demethylase, KDM6B, Lysine Demethylase 6B

Molecular Weight: 73.4 kDa

NCBI Accession: NP_001073893

Pathways: Warburg Effect