MBD1 Protein (Methyl-CpG-binding domain)

Catalog No. ABIN7939271

This Recombinant MBD1 protein is produced in Escherichia coli (E. coli).

Quick Overview for MBD1 Protein (Methyl-CpG-binding domain) (ABIN7939271)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: Methyl-CpG-binding domain

Purpose: Recombinant MBD1-MBD protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant MBD1-MBD protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1

Background:

Protein Background: MBD1 (Methyl-CpG-binding domain protein 1) is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Short Description: Recombinant MBD1-MBD protein that includes amino acids 1-70 of human MBD1 protein (accession number NP_056671.2) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 10.11 kDa.

Aliases: Methyl-CpG-binding domain protein 1, CXXC3, PCM1, Protein Containing Methyl-CpG-Binding Domain 1