NEK2 Protein

Catalog No. ABIN7939373

This Recombinant NEK2 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for NEK2 Protein (ABIN7939373)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant NEK2 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant NEK2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background:

Protein Background: NEK2 (NIMA Related Kinase 2) is a protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. NEK2 regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes and regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. NEK2 is involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1 and plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. It is also essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis.

Short Description: Recombinant NEK2 protein was expressed in baculovirus expression system as the full-length protein (accession number NP_002488.1) with a N-terminal FLAG tag. The molecular weight of the protein is 53 kDa.

Aliases: NIMA Related Kinase 2

Pathways: M Phase