PD-1 Protein (AA 25-167)

Catalog No. ABIN7939455

This Recombinant PD-1 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PD-1 Protein (AA 25-167) (ABIN7939455)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Product Details

Protein Characteristics: AA 25-167

Purpose: Recombinant PDCD1 / PD1 (25-167) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PDCD1 / PD1 (25-167) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1 & PD1

Background:

Protein Background: Programmed cell death protein 1 (PDCD1), inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. PDCD1 delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. PDCD1 suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.

Short Description: Recombinant PDCD1 / PD1 (25-167) protein that includes amino acids 25-167 of human PD1 protein (accession number NP_005009.2) was expressed in a baculovirus expression system, and contains an C-terminal FLAG tag. The molecular weight of the protein is 17.8 kDa.

Aliases: Programmed cell death protein 1

Pathways: Cancer Immune Checkpoints