PKC alpha Protein

Catalog No. ABIN7939463

Recombinant PKC alpha protein expressed in Escherichia coli (E. coli).

Quick Overview for PKC alpha Protein (ABIN7939463)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant PRKCA protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PRKCA protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA

Background:

Protein Background: PRKCA (Protein kinase C alpha type) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Short Description: Recombinant PRKCA protein was expressed in a baculovirus expression system as the full length protein (accession number NP_002728.2) with an N-terminal FLAG tag. The molecular weight of PRKCA is 78.02 kDa.

Aliases: Protein Kinase C alpha type, PKCA, PRKACA, PKC-A, PKCalpha

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling