PKC eta Protein

Catalog No. ABIN7939470

This Recombinant PKC eta protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PKC eta Protein (ABIN7939470)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant PRKCH protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PRKCH protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: PRKCH

Background:

Protein Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCH (Protein Kinase C Eta), a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. PRKCH can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). Diseases associated with PRKCH include Stroke, Ischemic and Glioblastoma.

Short Description: Recombinant PRKCH protein was expressed in baculovirus expression system as the full length protein (accession number NP_006246.2) with a N-terminal FLAG tag. The molecular weight of the protein is 79.1 kDa.

Aliases: Protein Kinase C Eta, PKC Eta

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling