PKN2 Protein

Catalog No. ABIN7939478

Recombinant PKN2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for PKN2 Protein (ABIN7939478)

Target: PKN2 (Protein Kinase N2 (PKN2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Product Details

Purpose: Recombinant PKN2 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PKN2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PKN2 (Protein Kinase N2 (PKN2))

Alternative Name: PKN2

Background:

Protein Background: PKN2 (Protein Kinase N2), a PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. PKN2 plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. This protein phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin and phosphorylates HDAC5, therefore lead to impair HDAC5 import. PKN2 is required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. It also stimulates FYN kinase activity which is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. PKN2 regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion and inhibits Akt pro-survival-induced kinase activity.

Short Description: Recombinant PKN2 protein was expressed in baculovirus expression system as the full length protein (accession number NP_001307638.1) with a N-terminal FLAG tag. The molecular weight of the protein is 111.6 kDa.

Aliases: Protein Kinase N2

Pathways: Cell-Cell Junction Organization