SUMO2 Protein

Catalog No. ABIN7939589

This Recombinant SUMO2 protein is produced in Escherichia coli (E. coli).

Quick Overview for SUMO2 Protein (ABIN7939589)

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant SUMO2 protein - biotinylated

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant SUMO2 protein - biotinylated is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: SUMO2

Background:

Protein Background: SUMO2 (Small ubiquitin-related modifier 2) is a ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post translational modification on lysine residues of proteins plays a crucial role in several cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX.

Short Description: Recombinant SUMO2 protein - biotinylated that includes full length of human SUMO2 protein (accession number NP_008868.3) was expressed in E. coli and contains an N-terminal His & Avi tag with a molecular weight of 15.12 kDa. This protein has been modified by biotin in vivo. The purity of the protein is ≥ 70 % by SDS-PAGE.

Aliases: Small ubiquitin-related modifier 2, SMT3B, SMT3 Homolog 2

Pathways: Methionine Biosynthetic Process