AMPK alpha Protein (Complex)

Catalog No. ABIN7939596

This Recombinant AMPK alpha protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for AMPK alpha Protein (Complex) (ABIN7939596)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant AMPK Complex (A2+B2+G1) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Recombinant AMPK Complex (A2+B2+G1) Complex is in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100 and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: AMPK

Background:

Protein Background: AMPK is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. It also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE and regulates fatty acid and cholesterol synthesis by phosphorylating acetylCoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. It also regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK is a heterotrimer consisting of an alpha catalytic subunit (PRKAA1 or PRKAA2), and non-catalytic beta (PRKAB1 or PRKAB2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Short Description: Recombinant AMPK Complex (A2+B2+G1) that includes full length human PRKAA2 protein (accession number NP_006243.2) with a N-terminal FLAG tag and full length human PRKAB2 protein (accession number NP_005390.1) without tag and full length human PRKAG1 protein (accession number NP_002724.1) without tag was expressed in Hi5 cells. The molecular weights of PRKAA2, PRKAB2, PRKAG1 are 63.6 kDa, 30.3 kDa and 37.6 kDa, respectively.

Aliases: AMP-activated protein kinase

Pathways: Warburg Effect